Microtubules effectors

Working on cytoskeleton effectors? You need precise, reliable, and innovative assay to characterize the effect of your molecules on microtubules?
Ask us! With more than 20 years of expertise in cytoskeleton, we have solutions for you.

The Cytoskeleton Experts

Does your molecules interfere with tubulin polymerization?

Analyze the effects of your compounds on tubulin polymerization (microtubules formation) with our tubulin polymerization assay service. Do not guess: fast & reliable result!

Microtubules polymerization is followed by spectrophotometry at ΔA350 over one hour in presence of your compounds. Measurements are performed in triplicate and in parallel with control compounds: taxol (a stabilizing drug) and colchicine (a destabilizing drug). Thanks to this, the effect of your compounds will be accuratly determined.

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And we can do a lot more!

Visualize microtubules after polymerization

Discard any artifacts by visualizing the microtubules at the end of the polymerization assay. Immunofluorescence enables to visually determine the state of microtubules and to compare with reference compounds.

Confirm the binding site of your molecules

The colchicine displacement assay takes advantage of the colchicine fluorescence upon binding to β-tubulin. Binding of another molecule (non-fluorescent) to the colchicine binding site will displace the colchicine and the fluorescence intensity will therfore decrease.

Contact us! We would be happy to discuss your project with you.

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Visualization of the microtubules in living cells

Visualize the effects of your compound on the microtubule cytoskeleton and the mitotic spindle with stunning high quality immufluorescence images. As cytoskeleton specialists, we’ll detect, document and interpret the phenotype induced by your lead compounds.

More about microtubules?

Microtubules are critical components of the cytoskeleton and play several major roles in cells. Tubulin is a well-know target in oncology as they are of particular importance during mitosis, when they form a mitotic spindle, a bipolar array of microtubules and microtubule-binding proteins with chromosomes in the middle. Microtubules are composed of tubulin, a heterodimer of α and β subunits with an apparent molecular mass of 110 kDa. In the presence of GTP, the α and β tubulin heterodimers can polymerize (in-vivo and in-vitro) to form microtubules. Microtubules have a crucial role in facilitating long-distance transport of both proteins and organelles. In neurons, impaired fast axonal transport (FAT) is a hallmarks of tautopathies. Microtubules stabilization reversed FAT (Zhang B, et al.).